Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17362] to GATA1 - ChIP Grade
Suitable for: IHC-P, WB, ChIP, IP
Reacts with: Human

Product name

Anti-GATA1 antibody [EPR17362] - ChIP Grade
See all GATA1 primary antibodies
Description

Rabbit monoclonal [EPR17362] to GATA1 - ChIP Grade
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, HEL and MOLT-4 whole cell lysates. IHC-P: Human colon and cervix carcinoma tissues. ICC/IF: K562 cells. IP: K562 whole cell extract. ChIP: Chromatin from K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17362
Isotype

IgG
Research areas

Western blot - Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

All lanes : Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544) at 1/10000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : HEL (Human bone marrow erythroleukemia) whole cell lysate
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 5 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 15 seconds

HeLa cells do not express GATA1. The expression profile observed in HeLa is consistent with the literature (PMID: 27010793, PMID: 17196618, PMID: 22235304, PMID: 20823267).

Blocking/Dilution buffer: 5% NFDM/TBST.
ChIP - Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

Chromatin was prepared from K562 (Human chronic myelogenous leukemia cells from bone marrow) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab181544 (blue), and 20µl of Anti rabbit IgG sepharose beads. 5μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
“pro” stands for promoter region, while “NC2” stands for negative control which is negative loci at the promoter region.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human cervical cancer is observed. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human colon stroma is observed. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

GATA1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab181544 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab181544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 µg (Input). Lane 2: ab181544 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181544 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)

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