Anti-GATA1 antibody (ab40847)
Key features and details
- Goat polyclonal to GATA1
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GATA1 antibody
See all GATA1 primary antibodies -
Description
Goat polyclonal to GATA1 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human GATA1 aa 65-76 (internal sequence).
Sequence:DAEAYRHSPVFQ
Database link: P15976
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GATA1 antibody (ab40847)All lanes : Anti-GATA1 antibody (ab40847) at 1 µg/ml
Lane 1 : K562 nuclear cell lysate
Lane 2 : Human Hippocampus lysate
Developed using the ECL technique.
Predicted band size: 43 kDaNegative Control: Human Hippocampus Lysate.
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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized HeLa cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). DAPI was used as a nuclear counterstain (blue) and actin filaments were stained with phalloidin (red).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Flow Cytometry - Anti-GATA1 antibody (ab40847)Flow cytometric analysis of paraformaldehyde fixed, 0.15% triton-permeablized K562 cells labelling GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody (1ug/ml) (blue). Primary incubation carried out for 1hr.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized NIH3T3 cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). Primary incubation was carried out for 1 hour. DAPI was used as a nuclear counterstain (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Western blot - Anti-GATA1 antibody (ab40847)Anti-GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).
Predicted band size: 43 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
