Anti-ZHX3 antibody (ab99353)
Key features and details
- Rabbit polyclonal to ZHX3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZHX3 antibody
See all ZHX3 primary antibodies -
Description
Rabbit polyclonal to ZHX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 906-956 of Human ZHX3 (NP_055850.1)
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZHX3 antibody (ab99353)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99353 at 1/1000 (0.2µg/ml). Detection: DAB.
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Western blot - Anti-ZHX3 antibody (ab99353)All lanes : Anti-ZHX3 antibody (ab99353) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ZHX3 antibody (ab99353)Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99353 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds.
