Anti-GATA1 antibody - N-terminal (ab173816)
Key features and details
- Rabbit polyclonal to GATA1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GATA1 antibody - N-terminal
See all GATA1 primary antibodies -
Description
Rabbit polyclonal to GATA1 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human GATA1. N-terminal truncated Human recombinant protein
Database link: P15976 -
Positive control
- NCCIT, NTERRA and K562 cell lysates; U2OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 69% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody - N-terminal (ab173816)
Immunofluorescent analysis of formalin-fixed, permeabilized U2OS cells, labeling GATA1 with ab173816 at 1/200 dilution (green, middle panel) . Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Nuclei (blue, left panel) were stained with Hoechst 33342 dye.
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Western blot - Anti-GATA1 antibody - N-terminal (ab173816)All lanes : Anti-GATA1 antibody - N-terminal (ab173816) at 1/500 dilution
Lane 1 : NCCIT whole cell lysate
Lane 2 : NTERRA whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-rabbit-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa4-20% Tris-HCl polyacrylamide gel.
