Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)
![Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)](https://www.abcam.com/ps/products/242/ab242195/Images/ab242195-331266-anti-erbb-2-antibody-cl0268-immunohistochemistry.jpg "Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)")
Key features and details
- Mouse monoclonal [CL0268] to ErbB2 / HER2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ErbB2 / HER2 antibody [CL0268]
See all ErbB2 / HER2 primary antibodies -
Description
Mouse monoclonal [CL0268] to ErbB2 / HER2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ErbB2/ HER2 aa 250-400.
Database link: P04626 -
Positive control
- IHC-P: HER2-postive human breast cancer tissue. WB: SK-BR-3 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL0268 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)
Paraffin-embedded human HER2-positive breast cancer tissue stained for ErbB 2 using ab242195 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)](https://www.abcam.com/ps/products/242/ab242195/Images/ab242195-331267-anti-erbb-2-antibody-cl0268-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)Negative control:
Paraffin-embedded human HER2-negative breast cancer tissue stained for ErbB 2 using ab242195 at 1/50 dilution in immunohistochemical analysis. No positive ErbB 2 staining as expected.
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![Western blot - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)](https://www.abcam.com/ps/products/242/ab242195/Images/ab242195-331268-anti-erbb-2-antibody-cl0268-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [CL0268] (ab242195)All lanes : Anti-ErbB2 / HER2 antibody [CL0268] (ab242195) at 1 µg/ml
Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate.
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 137 kDa
