Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8276] to Cytochrome P450 4A/CYP4A11
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276]
See all Cytochrome P450 4A/CYP4A11 primary antibodies
Description

Rabbit monoclonal [EPR8276] to Cytochrome P450 4A/CYP4A11
Host species

Rabbit
Specificity

The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Cytochrome P450 4A/CYP4A11 aa 50-150. The immunogen is homologous to human cytochrome P450 4A11 and 4A22.
Positive control
- Mouse liver, Rat liver, Human fetal kidney and fetal liver cell lysates. Human liver and kidney tissue.
General notes
This product was previously labelled as Cytochrome P450 4A

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8276
Isotype

IgG
Research areas

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (ab140635)

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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