Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)

Key features and details

  • Mouse monoclonal [1H10] to VLDL Receptor/VLDL-R
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-VLDL Receptor/VLDL-R antibody [1H10]
    See all VLDL Receptor/VLDL-R primary antibodies

  • Description

    Mouse monoclonal [1H10] to VLDL Receptor/VLDL-R

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human VLDL Receptor/VLDL-R (C terminal).

  • General notes

    Blocks apoE4 binding.

    Previously labelled as VLDL Receptor. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)
    Flow Cytometry - Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)

    Overlay histogram showing HEK293 cells stained with ab75591 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75591, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Flow Cytometry - Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591)
    Flow Cytometry - Anti-VLDL Receptor/VLDL-R antibody [1H10] (ab75591) This image is courtesy of an anonymous Abreview

    Flow cytometry analysis of Human THP-1 cell lines, staining VLDL Receptor/VLDL-R (red histogram) with ab75591.

    Cells were taken from suspension culture and washed with PBS/BSA before fixation. Cells were blocked and permeabilized in 10% donkey serum/0.3 M glycine/0.1% BSA/0.1% Tween-20 in PBS for 20 mins at room temperature. The sample was incubated with the primary antibody (2 µg/106 cells in 0.1% BSA/0.1% Tween-20 in PBS) for 30 minutes at 22°C. A FITC-conjugated donkey anti-mouse polyclonal IgG (30 µg/ml) was used as the secondary antibody.

    Gating Strategy: FSC/SSC

    See Abreview

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