Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4054] to CD168 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD168 antibody [EPR4054] - BSA and Azide free
    See all CD168 primary antibodies

  • Description

    Rabbit monoclonal [EPR4054] to CD168 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: MCF-7. IHC-P: Human gastric carcinoma tissue. Human testis tissue.

  • General notes

    ab271887 is the carrier-free version of ab124729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunoprecipitation - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified  ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • OI-RD Scanning - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    OI-RD Scanning - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

    Immunohistochemical analysis of paraffin-embedded human testis tissue using unpurified ab124729 at 1/50 - 1/100 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).

  • Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)
    Anti-CD168 antibody [EPR4054] - BSA and Azide free (ab271887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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