Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4054] to CD168 - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free
    See all CD168 primary antibodies

  • Description

    Rabbit monoclonal [EPR4054] to CD168 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Lysates of: MDA-MB-435, LnCaP, T47-D, RAW264.7, PC-12. Human testis tissue

  • General notes

    ab228482 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Western blot - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

    This WB data was generated using the same anti-CD168 antibody clone, EPR4054, in a different buffer formulation (cat# ab124729).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: CD168 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE.  Ab124729 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Immunoprecipitation - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

    ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified  ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).

  • OI-RD Scanning - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    OI-RD Scanning - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124729).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

    This IHC data was generated using the same anti-CD168 antibody clone, EPR4054, in a different buffer formulation (cat# ab124729).

    Immunohistochemical analysis of paraffin-embedded human testis tissue using  unpurified ab124729 at 1/50 - 1/100 dilution

  • Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)
    Anti-CD168 antibody [EPR4054] - Low endotoxin, Azide free (ab228482)

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