Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)
Key features and details
- Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker -
Description
Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: RabbitDoes not react with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Human TRA-1-81.
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Positive control
- WB: HUES7 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
Clonality
Monoclonal -
Clone number
TRA-1-81 -
Myeloma
P3x66Ag.8-Sp2/0 -
Isotype
IgM -
Research areas
Images
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Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 200-400 kDa
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary
Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 200-400 kDa
Exposure time: 3 minutes