Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14643] to CD163 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD163 antibody [EPR14643] - C-terminal
    See all CD163 primary antibodies

  • Description

    Rabbit monoclonal [EPR14643] to CD163 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, Human placenta, Human breast cancer and Human spleen lysates; Human liver and Human stomach tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CD163 with ab188571 at 1/500 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab188571 at 1/500. A prediluted HRP Polymer for Rabbit IgG was use as the secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)
    Western blot - Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)
    All lanes : Anti-GIPR antibody - N-terminal (ab188591) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human placenta lysate
    Lane 3 : Human breast cancer lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 125 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)
    Anti-CD163 antibody [EPR14643] - C-terminal (ab188571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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