Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
![Anti-ALDH7A1 antibody [EP1935Y] (ab53278)](https://www.abcam.com/ps/products/53/ab53278/Images/ab53278-66051-anti-aldh7a1-antibody-ep1935y-western-blot.png "Anti-ALDH7A1 antibody [EP1935Y] (ab53278)")
Key features and details
- Rabbit monoclonal [EP1935Y] to ALDH7A1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH7A1 antibody [EP1935Y]
See all ALDH7A1 primary antibodies -
Description
Rabbit monoclonal [EP1935Y] to ALDH7A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ALDH7A1 aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P49419 -
Positive control
- WB: Human liver lysate IHC-P: Human liver tissue
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1935Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/50000 dilution + Human liver lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)](https://www.abcam.com/ps/products/53/ab53278/Images/ab53278-262045-anti-aldh7a1-antibody-ep1935y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling ALDH7A1 with purified ab53278 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)](https://www.abcam.com/ps/products/53/ab53278/Images/ab53278-66050-anti-aldh7a1-antibody-ep1935y-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)ab53278, at a 1/100 dilution, staining ALDH7A1 in paraffin embedded human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
