Anti-ALDH7A1 antibody (ab236663)
Key features and details
- Rabbit polyclonal to ALDH7A1
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ALDH7A1 antibody
See all ALDH7A1 primary antibodies -
Description
Rabbit polyclonal to ALDH7A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human ALDH7A1 aa 350 to the C-terminus.
Database link: P49419 -
Positive control
- WB: HeLa, HepG2, HEK-293T and SH-SY5Y cell lysate; Rat liver and kidney lysate; Mouse liver and kidney lysate. IHC-P: Human kidney and liver tissue. ICC/IF: HeLa cells. IP: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALDH7A1 antibody (ab236663)All lanes : Anti-ALDH7A1 antibody (ab236663) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lane 7 : Mouse liver lysate
Lane 8 : Mouse kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab236663)Paraffin-embedded human kidney tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody (ab236663)Paraffin-embedded human liver tissue stained for ALDH7A1 using ab236663 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody (ab236663)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ALDH7A1 using ab236663 at a dilution of 1/133 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). -
Immunoprecipitation - Anti-ALDH7A1 antibody (ab236663)ALDH7A1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236663.
Lane 1: Rabbit control IgG instead of ab236663 in HeLa whole cell lysate.
Lane 2: 6 µg ab236663 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
