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Epigenetics and Nuclear Signaling Histones H3 Methylated

Alexa Fluor® 488 Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (ab201749)

Price and availability

308 236 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Alexa Fluor® 488 Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (ab201749)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [6F8-D9] to Histone H4 (di methyl K20, tri methyl K20)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 488 Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9]
    See all Histone H4 primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [6F8-D9] to Histone H4 (di methyl K20, tri methyl K20)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    6F8-D9
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (ab201749)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (ab201749)

    ab201749 staining Histone H4 (di methyl K20, tri methyl K20) in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201749 at 1/1250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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