Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Республика Казахстан, г.Нур-Султан, Иманова 18/2

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Cancer Invasion/microenvironment Apoptosis Caspases

Anti-Caspase-3 antibody [E87] (ab32351)

Price and availability

392 708 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Caspase-3 antibody [E87] (ab32351)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E87] to Caspase-3
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Product image
HRP Anti-Caspase-7 antibody [E22] (ab206039)
Product image
Anti-Caspase-8 antibody [ABM14C1] (ab220171)
Product image
Anti-Caspase-4 antibody [EPR20921-83] (ab238124)
Product image
Anti-pro Caspase-7 antibody [Y33] - BSA and Azide free (ab247219)

Overview

  • Product name

    Anti-Caspase-3 antibody [E87]
    See all Caspase-3 primary antibodies
  • Description

    Rabbit monoclonal [E87] to Caspase-3
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for the pro form and the p17 cleaved form of human Caspase-3.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Caspase-3 aa 50-150. The exact sequence is proprietary.
    Database link: P42574

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899); Wild-type HAP1 whole cell lysate; Ramos and HEK-293 cell lysates. IHC-P: Human tonsil and cervical carcinoma tissue. ICC/IF: Jurkat cells. Flow Cyt: HeLa and Ramos cells. IP: HeLa whole cell lysate.
  • General notes

    Rat reactivity: We have preliminary internal testing data indicating this antibody does not react with mouse caspase 3. Internal testing and customer reports also indicate non-reactivity with rat capsase 3, contrary to earlier positive reports. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E87
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution

    Lane 1 : DMSO control wild-type HAP1 whole cell lysate
    Lane 2 : Staurosporine treated wild-type HAP1 whole cell lysate
    Lane 3 : DMSO control CASP3 knockout HAP1 whole cell lysate
    Lane 4 : Staurosporine treated CASP3 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 32 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32351 observed at 31 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab32351 was shown to recognize Caspase 3 in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 Staurosporine Treated (CASP3) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HAP1 Staurosporine Treated (CASP3) knockout samples were subjected to SDS-PAGE. ab32351 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
    Immunohistochemical staining of paraffin embedded human tonsil with purified ab32351 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 antibody [E87] (ab32351)

    Immunofluorescence staining of Jurkat (human T cell leukemia cell line from peripheral blood) cells with purified ab32351 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32351 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [E87] (ab32351)

    Unpurified ab32351, at a 1/25 dilution, staining Capase-3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution (purified)

    Lane 1 : untreated Jurkat (human T cell leukemia cell line from peripheral blood)cell lysate
    Lane 2 : Jurkat treated with staurosporine

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 17,35 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    All lanes : Anti-Caspase-3 antibody [E87] (ab32351) at 1/5000 dilution (purified)

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Caspase-3 antibody [E87] (ab32351)
    Immunoprecipitation - Anti-Caspase-3 antibody [E87] (ab32351)

    ab32351 (purified) at 1/50 immunoprecipitating Cullin 1 in 10 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

  • Flow Cytometry - Anti-Caspase-3 antibody [E87] (ab32351)
    Flow Cytometry - Anti-Caspase-3 antibody [E87] (ab32351)

    Overlay histogram showing Ramos (human Burkitt's lymphoma cell line) cells fixed in 4% PFA and stained with purified ab32351 at a dilution of 1 in 180 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Western blot - Anti-Caspase-3 antibody [E87] (ab32351)
    Western blot - Anti-Caspase-3 antibody [E87] (ab32351)

    Carried out with unpurified antibody. Lane 1 = Caspase 3 protein (Active) (ab52314) 20 ng. Lane 2 = Caspase 9 protein (Active) (ab52203) 20 ng. Lane 3 = Extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with vehicle (ab136806) 20 ug. Lane 4 = Extract of HeLa cells treated with staurosporine (ab136806) 20 ug. SDS PAGE performed under reducing conditions (100 mM DTT Sample heated at 50°C). Primary : Lanes 1-4: Anti Caspase 3 antibody (ab32351) at 1:1000 dilution. Secondary : Lanes 1-4: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL for 10 min exposure. Blocking: in 5% Milk + PBS overnight at 4 C. Primary antibody: in 5% Milk + PBS for 2 hours at RT. Secondary antibody: in 5% Milk + PBS for 2 hours at RT. Predicted band size : 32 kDa and 17 kDa. Observed band size : 32 kDa and 17 kDa.

  • Flow Cytometry - Anti-Caspase-3 antibody [E87] (ab32351)
    Flow Cytometry - Anti-Caspase-3 antibody [E87] (ab32351)

    Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with unpurfied ab32351 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32351, 1/1000 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Anti-Caspase-3 antibody [E87] (ab32351)
    Anti-Caspase-3 antibody [E87] (ab32351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Caspase-3 antibody [E87] (ab32351)

  •  
  • Product image

    Anti-Caspase-3 antibody (ab13847)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-Caspase-3 antibody [E87] - BSA and Azide free (ab197202)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-Caspase-3 antibody [EPR18297] - BSA and Azide free (ab224271)

    Applications: IHC-P, IP, WB

  •  
  • Product image

    Anti-Caspase-3 antibody (ab90437)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-Caspase-3 antibody [ABM1C12] (ab208161)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-Caspase-3 antibody [EPR18297] (ab184787)

    Applications: IHC-P, IP, WB

  •  
  • Product image

    Anti-Caspase-3 antibody (ab44976)

    Applications: WB

  •  
  • Product image

    Biotin Anti-Caspase-3 antibody [E87] (ab195905)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-Caspase-3 antibody (ab4051)

    Applications: IHC-P, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.