Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)
![Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)](https://www.abcam.com/ps/products/250/ab250636/Images/ab250636-410355-anti-caspase-2-antibody-epr17881-bsa-and-azide-free-western-blot.jpg "Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17881] to Caspase-2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [EPR17881] to Caspase-2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250636 is the carrier-free version of ab182738. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250636 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17881 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)
This data was developed using ab182738, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg).
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg).
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab182738 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab182738 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)](https://www.abcam.com/ps/products/250/ab250636/Images/ab250636-407929-anti-caspase-2-antibody-epr17881-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate(Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hours
Lane 2 : Untreated Jurkat whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesThis data was developed using ab182738, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)](https://www.abcam.com/ps/products/250/ab250636/Images/ab250636-406026-anti-caspase-2-antibody-epr17881-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesThis data was developed using ab182738, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)](https://www.abcam.com/ps/products/250/ab250636/Images/ab250636-403506-anti-caspase-2-antibody-epr17881-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution + Human thymus tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesThis data was developed using ab182738, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)](https://www.abcam.com/ps/products/250/ab250636/Images/ab250636-5-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [EPR17881] - BSA and Azide free (ab250636)
