Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant rat PAI1 protein (Biotin) (ab170081)

Key features and details

Expression system: Escherichia coli
Purity: > 95% SDS-PAGE
Active: Yes
Suitable for: SDS-PAGE, Functional Studies

Product name

Recombinant rat PAI1 protein (Biotin)
See all PAI1 proteins and peptides
Biological activity

ab170081 typically contains 50 percent active PAI1.
Purity

> 95 % SDS-PAGE.
Expression system

Escherichia coli
Accession

P20961
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Rat
- Sequence
  
  SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTT AGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQ RDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISD LLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVP MMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAI TNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFS STQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPT EMVLDRSFLFVVRHNPTETILFMGQLMEP
- Predicted molecular weight
  
  43 kDa
- Amino acids
  
  24 to 402
Conjugation

Biotin

Alternative names
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
- Nexin plasminogen activator inhibitor type 1
- PAI
- PAI 1
- PAI-1
- PAI1_HUMAN
- PLANH1
- Plasminogen activator inhibitor 1
- Plasminogen activator inhibitor type 1
- Serine (or cysteine) proteinase inhibitor
- Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
- Serine proteinase inhibitor clade E member 1
- serpin
- Serpin E1
- Serpin peptidase inhibitor clade E
- Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
- Serpine 1
- SERPINE1
see all
Function

This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificity

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease

Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similarities

Belongs to the serpin family.
Post-translational
modifications

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
Cellular localization

Secreted.
Target information above from: UniProt accession P05121 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant rat PAI1 protein (Biotin) (ab170081)

SDS-PAGE analysis of ab170081.
Lane 1: 3µg ab170081 Reduced
Lane 2: Rat PAI1 (3µg) + Urokinase (15µg) Reduced
Lane 3: Molecular weight markers
Western blot - Recombinant rat PAI1 protein (Biotin) (ab170081)

Avidin-Alkaline Phosphatase Western Blot analysis of ab170081.
Lane 1: 0.1µg ab170081 Reduced
Lane 2: Rat PAI1 (3µg) + Urokinase (2µg) Reduced

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