Recombinant rat PAI1 protein (ab170082)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
-
Product name
Recombinant rat PAI1 protein
See all PAI1 proteins and peptides -
Biological activity
ab170082 is >60 percent active as determined by SDS PAGE. -
Purity
> 95 % SDS-PAGE.
The His tag allows for the immobilization of functionally active PAI1 onto surfaces such as metal chelate microtiter plates or Ni+2 resins. The purification conditions are gentle and result in an active fraction >99 percent pure and >60 percent active as determined by SDS PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Rat -
Sequence
SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTT AGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQ RDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISD LLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVP MMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAI TNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFS STQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPT EMVLDRSFLFVVRHNPTETILFMGQLMEP -
Predicted molecular weight
43 kDa including tags -
Amino acids
24 to 402 -
Tags
His tag N-Terminus
-
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 5.00
Constituents: 0.41% Sodium acetate, 0.03% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
SDS-PAGE - Recombinant rat PAI1 protein (ab170082)SDS-PAGE analysis of ab170082.
Lane 1: 3µg ab170082 Reduced
Lane 2: Rat PAI1 (3µg) + Urokinase (15µg) Reduced -
Western blot - Recombinant rat PAI1 protein (ab170082)Anti His tag Western Blot analysis of ab170082.Lane 1: 0.5µg ab170082 Reduced
Lane 2: Rat PAI1 (0.5µg) + Urokinase (7.5µg) Reduced
