Human uPAR ELISA Kit (ab246549)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human uPAR ELISA Kit
See all uPA Receptor/U-PAR kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 1.3% Inter-assay Sample n Mean SD CV% Supernatant 3 5.5% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 93% - 104% Urine 96 95% - 97% Serum 102 96% - 112% Hep Plasma 86 81% - 91% EDTA Plasma 95 93% - 96% Cit plasma 93 92% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human uPAR ELISA Kit (ab246549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of uPAR protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human uPAR with 21.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human uPAR Capture Antibody 1 x 600µl 10X Human uPAR Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human uPAR Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
Tissue specificity
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
Sequence similarities
Contains 3 UPAR/Ly6 domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD 87
- CD87
- CD87 antigen
see all -
Database links
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
Images
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Other - Human uPAR ELISA Kit (ab246549)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human uPAR Standard Curve ComparisonStandard Curve comparison between human uPAR SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human uPAR standard curve in Sample Diluent 50BS.The uPAR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human uPAR standard curve in Sample Diluent NS.The uPAR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native uPAR in human serum and plasma samples.The concentrations of uPAR were measured in duplicates, interpolated from the uPAR standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 5.34 ng/mL in serum, 4.30 ng/mL in plasma (citrate), 3.41 ng/mL in plasma (EDTA), and 4.81 ng/mL in plasma (heparin).
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Interpolated concentrations of native uPAR in human urine and cell culture supernatant samples.The concentrations of uPAR were measured in duplicates, interpolated from the uPAR standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 10% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 12.68 ng/mL in urine and 6.32 ng/mL in stimulated PBMC supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 6.38 ng/mL with a range of 3.42 – 8.79 ng/mL.
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Sandwich ELISA - Human uPAR ELISA Kit (ab246549)To learn more about the advantages of recombinant antibodies see here.
