Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)
![Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)](https://www.abcam.com/ps/products/234/ab234059/Images/ab234059-450840-anti-upa-antibody-ep6274-2-westernblot-pc-3-human.jpg "Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6274(2)] to uPA - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-uPA antibody [EP6274(2)] - BSA and Azide free
See all uPA primary antibodies -
Description
Rabbit monoclonal [EP6274(2)] to uPA - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: PC-3 cells; WB: PC-3 whole cell lysate.
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General notes
ab234059 is the carrier-free version of ab169754 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab234059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6274(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution (Purified) + PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?This data was developed using ab169754, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST
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![Immunocytochemistry - Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)](https://www.abcam.com/ps/products/234/ab234059/Images/ab234059-2-anti-upa-antibody-ep6274-2-immunocytochemistry-pc3-human.jpg)
Immunocytochemistry - Anti-uPA antibody [EP6274(2)] - BSA and Azide free (ab234059)This data was developed using ab169754 the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling uPA with Purified ab169754 at 1:50 dilution (3.78 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
