Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

Key features and details

  • Rabbit monoclonal [EP1630] to Cytokeratin 8 (phospho S431)
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]
    See all Cytokeratin 8 primary antibodies

  • Description

    Rabbit monoclonal [EP1630] to Cytokeratin 8 (phospho S431)

  • Host species

    Rabbit

  • Specificity

    ab109452 only detects Cytokeratin 8 phosphorylated at Serine 431

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa lysates, treated with FBS and Calyculin; Human ductal breast carcinoma and Human stomach adenocarcinoma tissues. IP: Calyculin A whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    All lanes : Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution

    Lane 1 : HeLa lysates, untreated
    Lane 2 : HeLa lysates, treated with FBS and Calyculin A

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 54 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

    ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Immunocytochemistry - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Cytokeratin 8 with purified ab109452 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.26 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

    Purified ab109452 at 1/20 dilution (0.5µg) immunoprecipitating Cytokeratin 8 in HeLa treated with Calyculin A whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10µg
    Lane 2 (+): ab109452 + HeLa treated with Calyculin A whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109452 in HeLa treated with Calyculin A whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

    ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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