Rabbit IL-21 ELISA Kit (ab273246)
Key features and details
- Sensitivity: 2.45 ng/ml
- Range: 2.458 ng/ml - 600 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Overview
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Product name
Rabbit IL-21 ELISA Kit
See all IL-21 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.45 ng/ml -
Range
2.458 ng/ml - 600 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 71.2 67% - 77% Plasma 71.55 67% - 82% Cell culture media 144.6 140% - 148% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rabbit -
Product overview
Rabbit IL-21 ELISA Kit (ab273246) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit IL-21 in serum (rabbit IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as an anti-coagulant for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for rabbit IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Rabbit IL-21 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit IL-21 Antibody 2 vials Rabbit IL-21 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CVID11
- IL 21
- IL-21
see all
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
