Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10360] to Factor XIIIa - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free
    See all Factor XIIIa primary antibodies

  • Description

    Rabbit monoclonal [EPR10360] to Factor XIIIa - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human Factor XIIIa. The exact sequence is proprietary.
    Database link: P00488

  • General notes

    Ab250089 is the carrier-free version of ab179444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250089 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Factor XIIIa with ab179444 at 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Western blot - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    All lanes : Anti-Factor XIIIa antibody [EPR10360] (ab179444) at 1/1000 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human placenta tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 83 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab179444, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Immunocytochemistry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U937 cells labeling Factor XIIIa with ab179444 at 1/50 (green). DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Flow Cytometry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    This data was developed using ab179444, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized U937 cells labeling Factor XIIIa with ab179444 at 1/10 (red) or a rabbit IgG (negative) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human fibrosarcoma tissue labeling Factor XIIIa with ab179444 at 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
    Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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