Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14000-76] to POC5 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-POC5 antibody [EPR14000-76] - N-terminal
    See all POC5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14000-76] to POC5 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Y79 and Human testis lysate. Human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling POC5 with ab188330 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Western blot - Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330) at 1/1000 dilution + Y79 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)
    Anti-POC5 antibody [EPR14000-76] - N-terminal (ab188330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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