Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: >= 98% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: Functional Studies, HPLC, SDS-PAGE

Product name

Recombinant rat G-CSF protein
See all G-CSF proteins and peptides
Biological activity

Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED₅₀ is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.
Purity

>= 98 % SDS-PAGE.
Endotoxin level
Expression system

Escherichia coli
Accession

NP_058800.1
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Rat
- Sequence
  
  MKKIPLLTVSSLPPSLPLPRSFLLKSLEQVRKIQARNTELLEQLCATYKL CHPEELVLFGHSLGIPKASLSSCSSQALQQTKCLSQLHSGLFLYQGLLQA LAGISSELAPTLDMLHLDVDNFATTIWQQMESLGVAPTVQPTQSTMPIFT SAFQRRAGGVLVTSYLQSFLETAHHALHHLPRPAQKHFPESLFISI
- Predicted molecular weight
  
  22 kDa
- Amino acids
  
  22 to 214

Our Abpromise guarantee covers the use of ab176083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

Functional Studies

HPLC

SDS-PAGE
Form

Lyophilized
Concentration information loading...

Stability and Storage

Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Constituent: 0.26% Sodium citrate

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Alternative names
- C17orf33
- Colony stimulating factor 3
- Colony stimulating factor 3 (granulocyte)
- CSF 3
- CSF beta
- CSF3
- CSF3_HUMAN
- CSF3OS
- Csfg
- Filgrastim
- G-CSF
- GCSA
- GCSF
- Granulocyte colony stimulating factor
- Granulocyte colony-stimulating factor
- Lenograstim
- Macrophage granulocyte inducer 2
- MGC45931
- MGI 2
- Pluripoietin
see all
Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Sequence similarities

Belongs to the IL-6 superfamily.
Post-translational
modifications

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Cellular localization

Secreted.
Target information above from: UniProt accession P09919 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols