Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant rat G-CSF protein (ab176083)

Key features and details

  • Expression system: Escherichia coli
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant rat G-CSF protein
    See all G-CSF proteins and peptides

  • Biological activity

    Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    NP_058800.1

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Sequence

      MKKIPLLTVSSLPPSLPLPRSFLLKSLEQVRKIQARNTELLEQLCATYKL CHPEELVLFGHSLGIPKASLSSCSSQALQQTKCLSQLHSGLFLYQGLLQA LAGISSELAPTLDMLHLDVDNFATTIWQQMESLGVAPTVQPTQSTMPIFT SAFQRRAGGVLVTSYLQSFLETAHHALHHLPRPAQKHFPESLFISI

    • Predicted molecular weight

      22 kDa

    • Amino acids

      22 to 214

    • Specifications

    Our Abpromise guarantee covers the use of ab176083 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituent: 0.26% Sodium citrate

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

    General Info

    • Alternative names

      • C17orf33
      • Colony stimulating factor 3
      • Colony stimulating factor 3 (granulocyte)
      • CSF 3
      • CSF beta
      • CSF3
      • CSF3_HUMAN
      • CSF3OS
      • Csfg
      • Filgrastim
      • G-CSF
      • GCSA
      • GCSF
      • Granulocyte colony stimulating factor
      • Granulocyte colony-stimulating factor
      • Lenograstim
      • Macrophage granulocyte inducer 2
      • MGC45931
      • MGI 2
      • Pluripoietin
      see all

    • Function

      Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

    • Sequence similarities

      Belongs to the IL-6 superfamily.

    • Post-translational
      modifications

      O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab176083? Please let us know so that we can cite the reference in this datasheet.

ab176083 has not yet been referenced specifically in any publications.

Preparation and Storage

  • C17orf33
  • Colony stimulating factor 3
  • Colony stimulating factor 3 (granulocyte)

Images

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