Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 95% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: Functional Studies, SDS-PAGE

Product name

Recombinant human G-CSF protein
See all G-CSF proteins and peptides
Biological activity

The ED50, determined by the dose-dependent stimulation of the proliferation of murine NSF-60 cells, is 7 units/mg.
Purity

> 95 % SDS-PAGE.
Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.
Endotoxin level
Expression system

Escherichia coli
Protein length

Full length protein
Animal free

No
Nature

Recombinant
- Species
  
  Human
- Sequence
  
  Human G-CSF is a 18.7 kDa protein containing 174 amino acid residues: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLG IPWAPLSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG P TLDTLQLDV ADFATTIWQQMEELGMAPAL QPTQGAMPAF ASAFQRRA GG VLVASHLQSF LEVSYRVLRH LAQP

Related Products
- Anti-G-CSF antibody (ab9691)

Our Abpromise guarantee covers the use of ab9692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

Functional Studies

SDS-PAGE
Form

Lyophilized
Additional notes

The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.

Alternative names
- C17orf33
- Colony stimulating factor 3
- Colony stimulating factor 3 (granulocyte)
- CSF 3
- CSF beta
- CSF3
- CSF3_HUMAN
- CSF3OS
- Csfg
- Filgrastim
- G-CSF
- GCSA
- GCSF
- Granulocyte colony stimulating factor
- Granulocyte colony-stimulating factor
- Lenograstim
- Macrophage granulocyte inducer 2
- MGC45931
- MGI 2
- Pluripoietin
see all
Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Sequence similarities

Belongs to the IL-6 superfamily.
Post-translational
modifications

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Cellular localization

Secreted.
Target information above from: UniProt accession P09919 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab9692? Please let us know so that we can cite the reference in this datasheet.

ab9692 has not yet been referenced specifically in any publications.

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

For lot specific reconstitution information please contact our Scientific Support Team.