Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant rhesus monkey G-CSF protein (ab200305)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant rhesus monkey G-CSF protein
    See all G-CSF proteins and peptides

  • Biological activity

    The ED50 as calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.1 ng/mL,
    corresponding to a Specific Activity of 1.0 x 107 IU/mg.

  • Purity

    > 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    F7H1Q6

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rhesus monkey

    • Sequence

      TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEEL V LLRHSLGIP WAPLSSCPSQALQLTGCLSQLHSSLFLYQGLLQALEGIS PELSPTLDT LQLDIADFAT TIWQQMEDLGMAPALQPTQGAMPAFTS AFQRRAGGVLVASHLQRF LELAYRVLR HLAQS

    • Predicted molecular weight

      19 kDa

    • Amino acids

      31 to 207

    • Specifications

    Our Abpromise guarantee covers the use of ab200305 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

    General Info

    • Alternative names

      • C17orf33
      • Colony stimulating factor 3
      • Colony stimulating factor 3 (granulocyte)
      • CSF 3
      • CSF beta
      • CSF3
      • CSF3_HUMAN
      • CSF3OS
      • Csfg
      • Filgrastim
      • G-CSF
      • GCSA
      • GCSF
      • Granulocyte colony stimulating factor
      • Granulocyte colony-stimulating factor
      • Lenograstim
      • Macrophage granulocyte inducer 2
      • MGC45931
      • MGI 2
      • Pluripoietin
      see all

    • Function

      Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

    • Sequence similarities

      Belongs to the IL-6 superfamily.

    • Post-translational
      modifications

      O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab200305? Please let us know so that we can cite the reference in this datasheet.

ab200305 has not yet been referenced specifically in any publications.

Preparation and Storage

  • C17orf33
  • Colony stimulating factor 3
  • Colony stimulating factor 3 (granulocyte)

Images

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