Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 98% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: SDS-PAGE, Functional Studies

Product name

Recombinant rhesus monkey G-CSF protein
See all G-CSF proteins and peptides
Biological activity

The ED₅₀ as calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.1 ng/mL,
corresponding to a Specific Activity of 1.0 x 10⁷ IU/mg.
Purity

> 98 % SDS-PAGE.
Endotoxin level
Expression system

Escherichia coli
Accession

F7H1Q6
Protein length

Protein fragment
Animal free

No
Nature

Recombinant
- Species
  
  Rhesus monkey
- Sequence
  
  TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEEL V LLRHSLGIP WAPLSSCPSQALQLTGCLSQLHSSLFLYQGLLQALEGIS PELSPTLDT LQLDIADFAT TIWQQMEDLGMAPALQPTQGAMPAFTS AFQRRAGGVLVASHLQRF LELAYRVLR HLAQS
- Predicted molecular weight
  
  19 kDa
- Amino acids
  
  31 to 207

Our Abpromise guarantee covers the use of ab200305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

Functional Studies
Form

Lyophilized
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituent: 100% PBS

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

Alternative names
- C17orf33
- Colony stimulating factor 3
- Colony stimulating factor 3 (granulocyte)
- CSF 3
- CSF beta
- CSF3
- CSF3_HUMAN
- CSF3OS
- Csfg
- Filgrastim
- G-CSF
- GCSA
- GCSF
- Granulocyte colony stimulating factor
- Granulocyte colony-stimulating factor
- Lenograstim
- Macrophage granulocyte inducer 2
- MGC45931
- MGI 2
- Pluripoietin
see all
Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Sequence similarities

Belongs to the IL-6 superfamily.
Post-translational
modifications

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Cellular localization

Secreted.
Target information above from: UniProt accession P09919 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols