Recombinant Human KRAS (mutated Q61H) protein (ab96817)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE, WB
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Product name
Recombinant Human KRAS (mutated Q61H) protein
See all KRAS proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab96817 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKC -
Predicted molecular weight
23 kDa including tags -
Amino acids
1 to 185 -
Modifications
mutated Q61H -
Tags
His tag N-Terminus -
Additional sequence information
Corresponds to K-Ras4B (Isoform 2B)
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chloride
Images
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Mass Spectrometry - Recombinant Human KRAS (mutated Q61H) protein (ab96817)
MALTI-TOF result: of 23253.422 Da for Human KRAS (mutated Q61H) protein.
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SDS-PAGE - Recombinant Human KRAS (mutated Q61H) protein (ab96817)15% SDS-PAGE showing ab96817 at approximately 23.2 kDa (3 µg).
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Western blot - Recombinant Human KRAS (mutated Q61H) protein (ab96817)This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab84573 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
