Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAC3 antibody [EPR6680] (ab129062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6680] to RAC3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAC3 antibody [EPR6680]
    See all RAC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR6680] to RAC3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RAC3 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: Human brain, Mouse brain, and Rat brain lysates. IHC-P: human cerebrum, mouse cerebrum, and rat cerebrum tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling RAC3 with Purified ab129062 at 1:100 dilution (2.64 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    We are unsure how to define the extra bands.

  • Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (Purified) + Human brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : Fetal brain lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC3 antibody [EPR6680] (ab129062)

    ab129062 (unpurified) at 1/50 dilution staining RAC3 in paraffin-embedded Human human breast carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-RAC3 antibody [EPR6680] (ab129062)
    OI-RD Scanning - Anti-RAC3 antibody [EPR6680] (ab129062)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    Western blot - Anti-RAC3 antibody [EPR6680] (ab129062)
    All lanes : Anti-RAC3 antibody [EPR6680] (ab129062) at 1/2000 dilution (unpurified)

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with Rac1 peptide
    Lane 3 : 293 cell extract with Rac2 peptide
    Lane 4 : 293 cell extract with Rac3 peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 21 kDa

  • Anti-RAC3 antibody [EPR6680] (ab129062)
    Anti-RAC3 antibody [EPR6680] (ab129062)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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