Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RALA antibody [EPR6468] (ab126627)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6468] to RALA
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-RALA antibody [EPR6468]
    See all RALA primary antibodies

  • Description

    Rabbit monoclonal [EPR6468] to RALA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RALA aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, MCF7, MOLT4, BxPC3 and HepG2 whole cell lysate (ab7900). Flow Cyt: MCF7 cells. IHC-P: Human breast carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RALA antibody [EPR6468] (ab126627)
    Western blot - Anti-RALA antibody [EPR6468] (ab126627)
    All lanes : Anti-RALA antibody [EPR6468] (ab126627) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RALA knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 24 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab126627 observed at 25 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab126627 Anti-RALA antibody [EPR6468] was shown to specifically react with RALA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265092 (knockout cell lysate ab258165) was used. Wild-type and RALA knockout samples were subjected to SDS-PAGE. ab126627 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-RALA antibody [EPR6468] (ab126627)
    Western blot - Anti-RALA antibody [EPR6468] (ab126627)
    All lanes : Anti-RALA antibody [EPR6468] (ab126627) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : BxPC3 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 24 kDa

  • Flow Cytometry - Anti-RALA antibody [EPR6468] (ab126627)
    Flow Cytometry - Anti-RALA antibody [EPR6468] (ab126627)
    Overlay histogram showing MCF7 cells stained with ab126627 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126627, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody [EPR6468] (ab126627)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody [EPR6468] (ab126627)

    ab126627, at 1/100, staining RALA in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RALA antibody [EPR6468] (ab126627)
    Anti-RALA antibody [EPR6468] (ab126627)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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