Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)
Key features and details
- Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
Overview
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Product name
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-P, ICC, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?