Recombinant human KIAA1549 + BRAF protein (ab204214)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human KIAA1549 + BRAF protein
See all KIAA1549 + BRAF proteins and peptides -
Biological activity
The specific activity of ab204214 was determined to be 750 nmol/min/mg.
-
Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Human -
Sequence
DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSS SSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQ WCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSN NIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDK NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSK VRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASE PSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH -
Predicted molecular weight
118 kDa including tags -
Amino acids
381 to 766 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_004333.
-
Amino Acid Sequence 2
-
Species
Human -
Sequence
YWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDIL IIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADST VSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHV DRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQ TALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQ MQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRL ITTDSDGTYRRPPGVHNSAYIGCP -
Amino acids
1320 to 1643 -
Additional sequence information
NM_001164665.
-
Preparation and Storage
-
Alternative names
- BRAF1
- p94
- Proto-oncogene B-Raf
see all -
Relevance
BRAF: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. -
Cellular localization
BRAF: Cell Membrane, Cytoplasmic and Nuclear; KIAA1549: Membrane; Multi-pass membrane protein
Images
-
Functional Studies - Recombinant human KIAA1549 + BRAF protein (ab204214)
Kinase assay showing the specific activity of ab204214 as 750 nmol/min/mg.
-
SDS-PAGE - Recombinant human KIAA1549 + BRAF protein (ab204214)SDS-PAGE analysis of ab204214.
Approximate MWt: 118 kDa.
