Anti-ASPP1 antibody (ab51831)
Key features and details
- Rabbit polyclonal to ASPP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASPP1 antibody
See all ASPP1 primary antibodies -
Description
Rabbit polyclonal to ASPP1 -
Host species
Rabbit -
Specificity
Minimal reactivity occurs against ASPP2. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to an internal sequence of ASPP1 (Human)
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Positive control
- MCF7 cells extracts
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ASPP1 antibody (ab51831)All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilution
Lane 1 : Non-transfected control
Lane 2 : MCF7 cells over expressing ASPP1
Lane 3 : MCF7 cells overexpressing ASPP2
Predicted band size: 121 kDa
Observed band size: 120,50 kDa why is the actual band size different from the predicted?
Additional bands at: 121 kDa. We are unsure as to the identity of these extra bands.
Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1/1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. -
Immunocytochemistry/ Immunofluorescence - Anti-ASPP1 antibody (ab51831)ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
