Human Thyroid Peroxidase/TPO ELISA Kit (ab277455)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thyroid Peroxidase/TPO ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107.4 72% - 144% Plasma 135.2 122% - 148% Cell culture media 136.7 123% - 148% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Thyroid Peroxidase/TPO ELISA Kit (ab277455) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thyroid Peroxidase/TPO in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay), and cell culture supernatants.
This assay employs an antibody specific for Human Thyroid Peroxidase/TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Thyroid Peroxidase/TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Thyroid Peroxidase/TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Thyroid Peroxidase/TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human Thyroid Peroxidase/TPO Antibody 2 vials Anti-Human Thyroid Peroxidase TPO coated Microplate 1 unit Human Thyroid Peroxidase/TPO standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). -
Pathway
Hormone biosynthesis; thyroid hormone biosynthesis. -
Involvement in disease
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsGlycosylated.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part. -
Cellular localization
Membrane and Cell surface. - Information by UniProt
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Alternative names
- MSA
- PERT_HUMAN
- TDH2A
see all -
Database links
- Entrez Gene: 7173 Human
- Omim: 606765 Human
- SwissProt: P07202 Human
- Unigene: 467554 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.