Recombinant human CD40 protein (Fc Chimera Active) (ab83917)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
-
Product name
Recombinant human CD40 protein (Fc Chimera Active)
See all CD40 proteins and peptides -
Biological activity
Activity: The ED50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
Theoretical Sequence EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDT WNR ETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVL HRSC SPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLV VQQAG TNKTDVVCGPQDRLRRSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG PSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQ YNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVY TLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDG SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Amino acids
21 to 193 -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
-
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: PBS, 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
-
Functional Studies - Recombinant human CD40 protein (Fc Chimera Active) (ab83917)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
SDS-PAGE - Recombinant human CD40 protein (Fc Chimera Active) (ab83917)1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83917
Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie. -
Western blot - Recombinant human CD40 protein (Fc Chimera Active) (ab83917)Anti-CD40 antibody (ab13545) at 1/1000 dilution +Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
