Recombinant mouse CD40 protein (Active) (ab220566)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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Functional Studies - Recombinant mouse CD40 protein (Active) (ab220566)
Immobilized ab220566 at 5μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tag with a linear range of 1-16 ng/mL.
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SDS-PAGE - Recombinant mouse CD40 protein (Active) (ab220566)SDS-PAGE analysis of reduced ab220566 stained overnight with Coomassie Blue.
The protein migrates as 30 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
