Recombinant human CD40 protein (Active) (ab155710)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C.
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human CD40 protein (ab155710)
SDS-PAGE of reduced ab155710 stained overnight with Coomassie Blue.
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ELISA - Recombinant human CD40 protein (ab155710)Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
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Functional Studies - Recombinant human CD40 protein (ab155710)Ab155710 at 0.1 µg/ml can bind to 293T cells overexpressing human CD40L as determined by FACS analysis.
