Human ICOS ELISA Kit (ab267638)
Key features and details
- Sensitivity: 1.2 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICOS ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84.1 76% - 94% Plasma 81.74 77% - 86% Cell culture media 102 97% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ICOS ELISA Kit is designed for the quantitative determination of ICOS in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human ICOS coated on a 96-well plate. Standards and samples are pipetted into the wells and ICOS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ICOS antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICOS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human ICOS coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human ICOS Detection Antibody 2 vials Human ICOS Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. -
Tissue specificity
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. -
Involvement in disease
Immunodeficiency, common variable, 1 -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- Activation inducible lymphocyte immunomediatory molecule
- Activation-inducible lymphocyte immunomediatory molecule
- AILIM
see all -
Database links
- Entrez Gene: 29851 Human
- Omim: 604558 Human
- SwissProt: Q9Y6W8 Human
- Unigene: 56247 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.