Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD45 antibody [PTPRC/1461] (ab218503)

Key features and details

Mouse monoclonal [PTPRC/1461] to CD45
Suitable for: ICC, Flow Cyt, IHC-P
Reacts with: Human
Isotype: IgG2a

Product name

Anti-CD45 antibody [PTPRC/1461]
See all CD45 primary antibodies
Description

Mouse monoclonal [PTPRC/1461] to CD45
Host species

Mouse
Tested applications

Suitable for: ICC, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Tissue, cells or virus corresponding to Human CD45. Human B-lymphocytes.
Database link: P08575
Positive control
- Flow Cyt: Jurkat cells. IHC-P: Human tonsil tissue. ICC: Jurkat cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

PTPRC/1461
Isotype

IgG2a
Light chain type

kappa
Research areas

Flow Cytometry - Anti-CD45 antibody [PTPRC/1461] (ab218503)

Flow Cytometric analysis of PFA-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells using ab218502 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PTPRC/1461] (ab218503)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD45 with ab218503 at 1 µg/mL for 30 minutes at RT.
Immunocytochemistry - Anti-CD45 antibody [PTPRC/1461] (ab218503)

Immunocytochemistry analysis of PFA-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling CD45 with ab218503 at 1 ug/ml followed by Goat anti-Mouse IgG-CF488 (Green). Nuclei are labelled with Redot (Red).

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