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Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (ab264073)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free
    See all OX40L/TNFSF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HUVEC cells. Flow Cyt: HeLa cells. IP: HUVEC cell lysate.

  • General notes

    ab264073 is the carrier-free version of ab263910. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264073 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)
    Immunoprecipitation - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)

    OX40L/TNFSF4 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg with ab263910 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263910 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg

    Lane 2: ab263910 IP in HUVEC whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263910 in HUVEC whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 20 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263910).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)
    Immunocytochemistry/ Immunofluorescence - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling OX40L/TNFSF4 with ab263910 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line.

    Negative control: HeLa (PMID: 1996093).

    ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263910).

  • Flow Cytometry - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)
    Flow Cytometry - Anti-CD252 antibody [EPR23155-317] - BSA and Azide free (ab264073)

    Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labeling OX40L/TNFSF4 with ab263910 at a 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 1996093).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263910).

  • Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (ab264073)
    Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (ab264073)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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