Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)
![Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)](https://www.abcam.com/ps/products/263/ab263910/Images/ab263910-3-anti-cd252-ox40l-antibody-epr23155-317-immunoprecipitation-huvec-human.jpg "Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4
- Suitable for: Flow Cyt, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-OX40L/TNFSF4 antibody [EPR23155-317]
See all OX40L/TNFSF4 primary antibodies -
Description
Rabbit monoclonal [EPR23155-317] to OX40L/TNFSF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HUVEC cells. Flow Cyt: HeLa cells. IP: HUVEC cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23155-317 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)
OX40L/TNFSF4 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg with ab263910 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263910 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10µg
Lane 2: ab263910 IP in HUVEC whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263910 in HUVEC whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)](https://www.abcam.com/ps/products/263/ab263910/Images/ab263910-2-anti-cd252-ox40l-antibody-epr23155-317-immunocytochemistry-huvec-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling OX40L/TNFSF4 with ab263910 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line.
Negative control: HeLa (PMID: 1996093).
ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Flow Cytometry - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)](https://www.abcam.com/ps/products/263/ab263910/Images/ab263910-1-anti-cd252-ox40l-antibody-epr23155-317-flowcytometry-huvec-hela-human.jpg)
Flow Cytometry - Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labeling OX40L/TNFSF4 with ab263910 at a 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 1996093).
Gated on viable cells.
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![Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)](https://www.abcam.com/ps/products/263/ab263910/Images/ab263910-7-benefits-of-recombinant-antibodies.png)
Anti-OX40L/TNFSF4 antibody [EPR23155-317] (ab263910)
