Anti-S100 antibody (ab34686)
Key features and details
- Rabbit polyclonal to S100
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-S100 antibody
See all S100 primary antibodies -
Description
Rabbit polyclonal to S100 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Dog, Non human primates, Mammals
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Immunogen
Full length native protein (purified): MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DADAVDKVMK ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS, corresponding to amino acids 1-94 of Cow S100 (alpha chain). A mixture of alpha alpha homodimers and alpha beta heterodimers was used.
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General notes
Please note that lot 296545 is supplied at 0.5mg/ml.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was purified from monospecific antiserum. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (ab34686) This image is courtesy of an anonymous Abreview
ab34686 staining S100 in Rat nerve tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/250 in PBS + 1x casein) for 1 hour at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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Western blot - Anti-S100 antibody (ab34686)All lanes : Anti-S100 antibody (ab34686) at 1/1000 dilution
Lane 1 : Bovine S100 monomer at 0.1 µg
Lane 2 : S100 from rat brain lysate at 35 µg
Secondary
All lanes : IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX
Observed band size: 11 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (ab34686)Ab34686, at a 1/500 dilution, staining S100 in mouse brain tissue by immunohistochemistry. The tissue was formalin fixed and embedded in paraffin. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (ab34686)Ab34686, at 1/500 dilution, staining S100 in formalin fixed and paraffin embedded human brain astrocyte tumor tissue by immunohistochemistry.
