Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19013] to S100 alpha - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-Fr, WB, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-S100 alpha antibody [EPR19013] - BSA and Azide free
    See all S100 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR19013] to S100 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-Fr, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle lysate; Mouse cerebral cortex, liver, heart and muscle lysates; RAW 264.7 whole cell lysate. IHC-P: Human cerebrum, tonsil and kidney tissues. IHC-Fr: Mouse brain, kidney and spleen tissues. IP: RAW 264.7 whole cell lysate.

  • General notes

    ab227580 is the carrier-free version of ab183979.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling S100 alpha with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on glial cells and negative on neuron cells of Human cerebrum was observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on glial cells of mouse cortex. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse kidney. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse spleen. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

  • Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    S100 alpha was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab183979 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183979 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

    Lane 2: ab183979 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A]  - Isotype Control (ab172730) instead of ab183979 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    This IHC data was generated using the same anti-S100 alpha antibody clone, EPR19013, in a different buffer formulation (cat# ab183979).

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive on germinal center of Human tonsil. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

    This IHC data was generated using the same anti-S100 alpha antibody clone, EPR19013, in a different buffer formulation (cat# ab183979).

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Part of kidney tubules were strongly staining. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)
    Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (ab227580)

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