Anti-S100 antibody, prediluted (ab948)
Key features and details
- Rabbit polyclonal to S100, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100 antibody, prediluted
See all S100 primary antibodies -
Description
Rabbit polyclonal to S100, prediluted -
Host species
Rabbit -
Specificity
This antibody reacts with cow S-100 protein A and B. It shows strong cross reactivity with human S-100 A and B. In the brain, it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin are also stained with this antibody. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Affinity purified S-100 protein isolated from cow brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Myeloma
unknown -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody, prediluted (ab948)
ab948 staining human normal skin. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus, at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend individual optmisation of all of the forementioned parameters.
