Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100P antibody [EPR6143] (ab133554)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6143] to S100P
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-S100P antibody [EPR6143]
    See all S100P primary antibodies

  • Description

    Rabbit monoclonal [EPR6143] to S100P

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human S100P aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P25815

  • Positive control

    • WB: SW480 and BxPC-3 cell lysates and human placenta tissue lysate. IHC-P: Human pancreatic adenocarcinoma and placenta tissues. ICC/IF: SW480 cells. IP: SW480 cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/200 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with unpurified ab133554 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)
    Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)

    Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/40. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with purified ab133554 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100P antibody [EPR6143] (ab133554)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic adenocarcinoma tissue labelling S100P with unpurified ab133554 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-S100P antibody [EPR6143] (ab133554)
    Flow Cytometry - Anti-S100P antibody [EPR6143] (ab133554)

    Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma ) cells labeling S100P with purified ab133554 at 1/800 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/1500 dilution (purified)

    Lane 1 : SW480 cell lysate
    Lane 2 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    Western blot - Anti-S100P antibody [EPR6143] (ab133554)
    All lanes : Anti-S100P antibody [EPR6143] (ab133554) at 1/1000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : Human placenta cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)
    Immunocytochemistry/ Immunofluorescence - Anti-S100P antibody [EPR6143] (ab133554)

    Immunocytochemistry/Immunofluorescence analysis of SW480 cells labelling S100P (green) with pruified ab133554 at 1/400. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
    Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)

    ab133554 (unpurified) at 1/20 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)
    Immunoprecipitation - Anti-S100P antibody [EPR6143] (ab133554)

    ab133554 (purified) at 1/80 immunoprecipitating S100P in SW480 cells. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • OI-RD Scanning - Anti-S100P antibody [EPR6143] (ab133554)
    OI-RD Scanning - Anti-S100P antibody [EPR6143] (ab133554)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-S100P antibody [EPR6143] (ab133554)
    Anti-S100P antibody [EPR6143] (ab133554)

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