Mouse Periostin ELISA Kit (ab243689)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 22.1 pg/ml
- Range: 93.75 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Periostin ELISA Kit
See all Periostin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 5 2.2% Inter-assay Sample n Mean SD CV% serum 3 4.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
22.1 pg/ml -
Range
93.75 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 92% - 102% Serum 100 95% - 106% Hep Plasma 107 104% - 108% EDTA Plasma 107 99% - 112% Cit plasma 102 98% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Periostin ELISA Kit (ab243689) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Periostin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Periostin with 22.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Periostin Capture Antibody 1 x 600µl 10X Mouse Periostin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse Periostin Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Fasciclin-I like
- MGC119510
- MGC119511
see all -
Database links
- Entrez Gene: 50706 Mouse
- SwissProt: Q62009 Mouse
- Unigene: 236067 Mouse
Images
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Other - Mouse Periostin ELISA Kit (ab243689)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Periostin Standard Curve Comparison.Standard Curve comparison between mouse Periostin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse Periostin standard curve in Sample Diluent NS.The Periostin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Periostin standard curve in Sample Diluent 50BS.The Periostin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Periostin in mouse serum and plasma samples.The concentrations of Periostin were measured in duplicates, interpolated from the Periostin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100, plasma (citrate) 1: 100, plasma (heparin) 1: 100, and plasma (EDTA) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 293.6 ng/mL in serum, 347.4 ng/mL in plasma (citrate), 219.8 ng/ml in plasma (heparin), and 161.1 ng/ml in plasma (EDTA).
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Sandwich ELISA - Mouse Periostin ELISA Kit (ab243689)To learn more about the advantages of recombinant antibodies see here.
