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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions

Mouse Periostin Antibody Pair - BSA and Azide free (ab256644)

Mouse Periostin Antibody Pair - BSA and Azide free (ab256644)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Periostin Antibody Pair - BSA and Azide free
    See all Periostin kits
  • Assay type

    ELISA set
  • Range

    93.75 pg/ml - 7000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Antibody Pair can be used to quantify Mouse Periostin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab243689), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse Periostin Capture Antibody (unconjugated) 1 x 100µg
    Mouse Periostin Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Cardiomyocyte precursors
  • Function

    Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity

    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities

    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications

    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q15063 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Fasciclin-I like
    • MGC119510
    • MGC119511
    • OSF 2
    • OSF-2
    • OSF2
    • Osteoblast specific factor
    • Osteoblast specific factor 2
    • Osteoblast specific factor 2 (fasciclin I like)
    • Osteoblast-specific factor 2
    • PDLPOSTN
    • Periodontal ligament specific periostin
    • Periostin
    • Periostin isoform thy2
    • Periostin isoform thy4
    • Periostin isoform thy6
    • Periostin isoform thy8
    • Periostin osteoblast specific factor
    • PN
    • POSTN
    • POSTN_HUMAN
    • RP11 412K4.1
    see all
  • Database links

    • Entrez Gene: 50706 Mouse
    • SwissProt: Q62009 Mouse
    • Unigene: 236067 Mouse

    Images

    • Sandwich ELISA - Mouse Periostin Antibody Pair - BSA and Azide free (ab256644)
      Sandwich ELISA - Mouse Periostin Antibody Pair - BSA and Azide free (ab256644)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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