Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin
Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Detyrosinated alpha Tubulin antibody [AA12]
See all Detyrosinated alpha Tubulin primary antibodies
Description

Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Detyrosinated alpha Tubulin.
Database link: Q71U36

Run BLAST with Run BLAST with
Positive control
- WB: Human, rat and mouse brain tissue lysate. Human and rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neuron cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

AA12
Isotype

IgG2a
Research areas

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

Exposure time: 7 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Negative control: Mouse heart (PMID:15899979).
Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

Lane 1 : Human hippocampus tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lane 4 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-4 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1, 2: 3 seconds; Lane 3, 4: 7 seconds.
Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Detyrosinated A Tubulin with ab254154 at 1/250 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3µm followed by image processing with maximum Z projection. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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