Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin
  • Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Detyrosinated alpha Tubulin antibody [AA12]
    See all Detyrosinated alpha Tubulin primary antibodies

  • Description

    Mouse monoclonal [AA12] to Detyrosinated alpha Tubulin

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Detyrosinated alpha Tubulin.
    Database link: Q71U36

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human, rat and mouse brain tissue lysate. Human and rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neuron cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    AA12

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa


    Exposure time: 7 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Mouse heart (PMID:15899979).

  • Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    All lanes : Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154) at 1/1000 dilution

    Lane 1 : Human hippocampus tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Rat hippocampus tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 3-4 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 3 seconds; Lane 3, 4: 7 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Cytoplasmic staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Detyrosinated A Tubulin with ab254154 at 1/250 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3µm followed by image processing with maximum Z projection. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)
    Anti-Detyrosinated alpha Tubulin antibody [AA12] (ab254154)

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