Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19934] to Periostin - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Periostin antibody [EPR19934] - BSA and Azide free
    See all Periostin primary antibodies

  • Description

    Rabbit monoclonal [EPR19934] to Periostin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes a different epitope of Periostin compared to ab219057.

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart, fetal kidney and breast cancer lysates. IHC-P: Human colon and lung cancer tissues. IP: Human breast cancer lysate.

  • General notes

    Ab223194 is the carrier-free version of ab219056. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223194 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    This data was developed using ab219056, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID: 26023718). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    This data was developed using ab219056, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID: 24273600). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    All lanes : Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 3 minutes


    This data was developed using ab219056, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution + Human breast cancer lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 3 minutes


    This data was developed using ab219056, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Immunoprecipitation - Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    This data was developed using ab219056, the same antibody clone in a different buffer formulation.Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Human breast cancer lysate 10 μg (Input). Lane 2: ab219056 IP in Human breast cancer lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)
    Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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