Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7697] to TFII I - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TFII I antibody [EPR7697] - BSA and Azide free
    See all TFII I primary antibodies

  • Description

    Rabbit monoclonal [EPR7697] to TFII I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248269 is the carrier-free version of ab129025 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248269 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    Western blot - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    All lanes : Anti-TFII I antibody [EPR7697] (ab129025) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 112 kDa
    Observed band size: 135-138 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab129025, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    This data was developed using ab129025, the same antibody clone in a different buffer formulation.ab129025, at 1/100 dilution staining TFII I in formalin fixed paraffin embedded Human colon tissue by immunohistochemistry Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    Immunocytochemistry - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    This data was developed using ab129025, the same antibody clone in a different buffer formulation.ab129025, at 1/100 dilution, staining TFII I in Hela cells by immunofluorescence
  • OI-RD Scanning - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    OI-RD Scanning - Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    This data was developed using ab129025, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)
    Anti-TFII I antibody [EPR7697] - BSA and Azide free (ab248269)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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